Marketplace Solutions. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Data Migration with Build Tool. PCR and RT-PCR are achieved by the use of the hot-start enzyme HotStarTaq Plus DNA Polymerase together with a specialized fast PCR or RT-PCR buffer. 2) If you only wanted to produce cDNA from a subset of all mRNA, then. ) to obtain cDNA. Sensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). 1 ml RNase-free water 205111 询价 The Omniscript RT Kit includes Omniscript Reverse Transcriptase, dNTPs and an optimized reaction buffer. This enzyme combination provides highly efficient and sensitive reverse transcription over a wide range of RNA templateLightning Experience. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. Both enzymes exhibit a high affinity for RNA, facilitating transcription through secondary structures that may inhibit other reverse transcriptases. この例には、再利用可能な. CNV qPCR Assays & Arrays;. This kit is ideal for analyzing low input RNA samples. This also inactivates the reverse transcriptases. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRRT reactions containing 4 ng (Sensiscript) or 16 ng of total RNA (Omniscript) generated DD patterns indistinguishable from the 200 ng pattern. The unique QuantiTect Reverse Transcription Kit provides a fast and convenient procedure for cDNA synthesis with integrated genomic DNA removal. Bioz Stars score: 86/100, based on 1 PubMed citations. 1 ml 1. Principle Omniscript Reverse Transcriptase (RT) has a high affinity for RNA, which enables efficient and sensitive reverse transcription of any template, leading to high yields of cDNA. Kim et al. Omniscript Reverse Transcription Kit (Qiagen). The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. The ABScript II One Step RT-qPCR Probe Kit is a convenient, ready-to-use kit with optimized components for use for reverse transcription and subsequent probe-based qPCR. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. Sensiscript RT is optimized for use with very small amounts of RNA (<50 ng), such as for single-cell RT-PCR or analysis of small biopsies. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. The unique combination of Omniscript and Sensiscript Reverse Transcriptases, with their high affinity for RNA templates, ensures highly efficient and sensitive transcription of RNA amounts from as little as 1 pg up to 2 µg. The ABScript II Reverse Transcriptase in the. Your reverse transcription may not be optimal. In reverse transcription, the cDNA is synthesized from the mRNA. CNV qPCR Assays & Arrays;. RT-qPCR was carried out inRNA concentration was photometrically determined, and 200 ng RNA was reverse transcribed using Omniscript RT kit with oligo dT Primer (Qiagen, Hilden, Germany). 4 Omniscript Reverse Transcriptase Handbook 04/99 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog No. RNA was quantified using a nanodrop spectrophotometer (Thermo) and reverse transcribed to cDNA with the Omniscript RT Kit (Qiagen, 205110) as per the manufacturer's instructions. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. RT-PCR; PCR for Difficult Targets; Fast PCR; Long PCR; Direct PCR; High Fidelity PCR; Hot Start PCR; Real-Time PCR; Routine PCR ReagentOmniscript Rt Kit, supplied by Qiagen, used in various techniques. 1 ml 4. Background Reverse transcription-polymerase chain reaction (RT-PCR) is a very sensitive technique to measure and to compare mRNA levels among samples. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript Reverse Transcription Kit Quick-Start Protocol (EN) Print Bookmark Share pdf 56KB English Format File size Language Download Get Adobe Reader Contact QIAGEN . However, the Sensiscript and PowerScript systems were more efficient for detecting high. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript Reverse Transcription Handbook 05/2004. The total volume per reaction is set to 20 μl. icon_0011_idea-s. The PCR products were resolved by electrophoresis on 1% agarose gels and visualized by ethidium bromide staining. Omniscript is a confident hand-lettered script without self-conscious style or idiosyncrasy. For 200 reverse-transcription reactions: 800 units Omniscript Reverse Transcriptase, 4 × 150 µL 10 × Buffer RT, 4 × 100 µL dNTP Mix (contains 5 mM each dNTP), 4 × 1. 95°C after the reverse transcription step. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Reverse transcription was applied with Omniscript RT Kit following manufacturer's protocol (Qiagen). The unique combination of Omniscript and Sensiscript Reverse Transcriptases, with their high affinity for RNA templates, ensures highly efficient and. 05). Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. A total of 1. Omniscript RT Kit (200) by Qiagen. , the OmniScript RT, an RNAse H+ RT, which is part of the QuantiTect Reverse Transcription Kit, as well as with RNase H. The OneStep Ahead RT Mix contains an RNase inhibitor and a blend of Omniscript® and Sensiscript® reverse transcriptases in combination with an RT-blocker for heat-mediated activation of the reverse-transcription step. There-fore, 2,500 copies (1 fg) was chosen as the relatively small. ZERO BIAS - scores, article reviews, protocol conditions and morePeripheral blood of individual 4 was collected into PAXgene Blood RNA tubes (PreAnalytiX), and RNA was isolated using the PAXgene Blood RNA Kit (Qiagen). Omniscript is designed for reverseFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThis study provides a complex overview of the influence of elements such as RT systems, qRTPCR chemistry, amount of background RNA, and transcript abundance on the efficiency of qRT-PCR. Global Contacts. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. 1x Buffer RT (dilute an aliquot of 10x Buffer RT accordingly using the RNase -free water supplied). The mRNA levels were quantified using a SYBR Premix Ex Kit (Bio‑Rad) with a CFX Real time PCR Detection Systems (Bio‑Rad). Simply add primers for fast and easy cDNA synthesis. Starting material Starting material QIAGEN now offers the first commercially available reverse transcriptases from a new source. It relies on the principle of reverse transcription which is a process ‘reverse’ to the transcription. It uses PrimeScript Reverse Transcriptase, which features excellent extension. Related products . For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. Content. , GC-rich. nos. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. アフィニティーが高い酵素による高収量cDNA わずか10コピーのテンプレートも高感度で検出 追加のRNase H分解は不要 煩わしいピペッティング操作なしの迅速で簡単な製法 Omniscript Reverse Transcription（RT）Kitは、各反応あたり50 ng. QIAsphere Digital Connectivity. The ABScript II One Step RT-qPCR Probe Kit is a convenient, ready-to-use kit with optimized components for use for reverse transcription and subsequent probe-based qPCR. 1 mL RNase-free. OmniScript にはモジュール形式のアーキテクチャが採用されている OmniScript の外観 (フロントエンド) は機能 (バックエンド) から切り離されています。 OmniScript では JavaScript Object Notation (JSON) メタデータ構造 (1)、スタイルシート (2)、データ (3) が互いに分離され. QIAGEN now offers the first commercially available reverse transcriptases from a new source. The easy one-tube setup and optimized components enable higher sensitivity. , Valencia, CA). show that ferrets are highly susceptible to SARS-CoV-2 infection and effectively transmit the virus by direct or indirect contact, recapitulating human infection. OmniScripts allow you to craft dynamic customer. Time step T0 contained the reverse transcription mix (Omniscript RT Kit) without performing reverse transcription, T1 after the reverse transcription, and T2 was after a multiple displacement. Differences Between OmniStudio and OmniStudio for Vlocity. Key words: Breast cancer, Formalin-fixed, Paraffin-embedded tissue, Quantitative RT-PCR,Reverse Transcription was performed using an OmniScript™ RT kit (QIAGEN) according to manufacturer’s instructions and using gene-specific primers to interrogate genes of interest. , M-MuLV or AMV), and the kind of approach used for RT (random hexamers, oligo-dT, or specific. # 205113 and 205213, respectively) reverse transcriptases. 1 ml RNase-free water 205111 询价Currently, almost all single-cell transcriptomic protocols rely on reverse transcription (RT). For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe outbreak of coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rapidly spreads, leading to a pandemic infection. Reverse transcription with AMV, MMLV, and Omniscript was performed at 37 °C, whereas with cAMV, Improm-II, and MMLVH it was performed at 45 °C, and with ThermoScript and SuperScript, it was performed at 50 °C. $1,382. The one-step format prevents contamination and allows for higher sensitivity. Mix carefully by vortexing for no more than 5 s, and centrifuge briefly to collect residual liquid from the sides of the tube. 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. g. 0 µl 10× RT buffer, 2. Place a nuclease-free PCR tube on ice and add approximately 2 μg of isolated total RNA. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Components. Taqman primers of selectin E. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. PCR primers were designed using online available primer design software from Thermo Fisher Scientific and were purchased from Thermo Fisher Scientific. 1fg/µl). 在终点法PCR中，用于RNA模板量为50 ng到2 μg的逆转录反应For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR高感度リアルタイム2ステップRT-PCRによる遺伝子発現解析 でcDNA合成とgDNA除去をわずか20分で実現する高速 cDNA合成のために、 5'および3'の転写産物の少ないcDNA合成でも高いcDNA収量 を実現。 RNA特異的なプライマーやプローブ ユニークなQuantiTect逆転写キットは. An OmniScript is an omnichannel customer engagement and a business tool built on the Salesforce platform. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe reverse transcription was achieved using Omniscript RT Kit (Qiagen), according to manufacturer’s instructions. There are several commercially available RT kits which differ mainly in the kind of enzyme they use, bacterial (rTth) or viral origin (e. EGFP cDNA were obtained with th e other RTases. Workflow for Configuring OmniAnalytics for a Third-Party Analytics. Bioz Stars score: 86/100, based on 1 PubMed citations. 1 mL RNase-free. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReverse transcription was carried out according to the manufacturer's protocol either with the Omniscript RT (Qiagen) or SuperScript II RT (Invitrogen). 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. For standard reverse transcription, with 50 ng to 2 µg of RNA per reaction, Omniscript RT provides optimal results for both high- and low-copy mRNA species. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. 1 ml RNase-free water. 1 ml 1. Omniscript RT system, whereas substantial copies of. Media Inquiries. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. 0 µl of 10 µM H-T11C (final concentration of. RT-PCR Assays. 1). It is also nice that the purification procedure (after labeling) is simple. Omniscript Reverse Transcriptase - For First-strand cDNA synthesis Two-tube RT-PCR. アフィニティーが高い酵素による高収量cDNA わずか10コピーのテンプレートも高感度で検出 追加のRNase H分解は不要 煩わしいピペッティング操作なしの迅速で簡単な製法 Omniscript Reverse Transcription（RT）Kitは、各反応あたり50 ng. was reverse transcribed to cDNA using the Omniscript RT kit (Qiagen) and random hexamer primers (Invitrogen; Ta˚strup, Denmark). High Ca2+ stimulated the upregulation (as assessed by RT-PCR) and the secretion of Wnt5a (assessed by Western blot), a. Total RNA was extracted with the use of Qiagen RNeasy Mini Kits for cells and with the use of TRIzol (Invitrogen) for tissue samples according to the manufacturer's protocol. First-strand cDNA was synthesized from 1 μg of high-quality total RNA using SuperScript II Reverse Transcriptase in accordance with the manufacturer’s instructions (QIAGEN, Omniscript RT). One microliter of kit-supplied enzyme mix-ture (combination of Omniscript Reverse. DSL Service has an attribute Download Spee d (ATT_RT_DS) and possible values are 7, 12, 20 and 40 Mbps. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor backward transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. The ABScript II Reverse Transcriptase in. Need Assistance?. During reverse transcription, chemically modified HotStarTaq DNA Polymerase is completely inactive and does not interfere with the reverse-transcriptase reaction. Omniscript RT system, whereas substantial copies of. We compared these five RTs for their utility in detecting trace transcripts by real-time PCR, ability to transcribe a long RNA and sensitivity. Kit contents: Qiagen Omniscript RT Kit, 50 rxns, RNA Template Sample, Reverse Transcription Enzyme Activity, High cDNA Yields Due to High-affinity Enzyme, Fast and Easy Procedure, For Reverse Transcription of 50ng to 2micro g. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. 1 ml 4. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. For reverse transcription of 50 ng to 2 μg RNA per reaction fork end-point PCR Omniscript Reverse Transcription（RT）Kitは、各反応あたり50 ngから2 µgのRNA量を用いた逆転写用に特別にデザインされています。 RNAへのアフィニティーが高いOmniscript Reverse Transcriptaseは、他の逆転写酵素と比較して非常に優れた性能を示し、低いコピー数の. Briefly, RNA was isolated using the RNAeasy Mini kit (Qiagen, Germany). 0 µl of Omniscript or Sensiscript RT (Qiagen, Santa Clarita, CA), 2. Format. 00. With no need for optimization, the Omniscript RT Kit makes reverse transcription virtually trouble-free. The Agilent 2100 Bioanalyzer (Agilent Technologies; Kista, Sweden) was used to confirm the integrity of the RNA. Omniscript RT Kit. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. PrimeScript RT Reagent Kit is designed to perform reverse transcription optimized for real-time RT-PCR. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. To minimize methodological errors resulting from. A5003), SuperScript® II and SuperScript® III (Invitrogen Cat. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. 2) If you only wanted to produce cDNA from a subset of all mRNA, then. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. It is a quantitative technique used to estimate gene copies. First Strand kit)? What is recommended? We have had a good experience with both types of Reverse Transcriptases, e. It is crucial to recognize and prevent potential issues with cDNA synthesis in order to maintain the reliability of experimental. First-strand cDNA synthesis was done using the Omniscript RT kit (Qiagen, Valencia, CA) as recommended by the manufacturer. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. RT activity is critical for many basic techniques including real-time and endpoint RT-PCR, labeled-probe generation for microarrays and cDNA cloning. Sensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). g. 205110, 205111and 205113) should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer. Please see the Omniscript Reverse Transcription Handbook for protocols. OmniStudio Standard. Quantitative real-time PCR (qPCR) was performed using the SYBR Premix Ex Taq (TaKaRa, Dalian, China) and the CFX96 TM Real-Time PCR. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRLevels of IL-6 mRNA in cells were evaluated by real-time reverse transcription and Omniscript Reverse Transcriptase Journal of Neuroinflammation. CNV qPCR Assays & Arrays;. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRThe Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. 1 ml 1. High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. . SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich. Vlocity Cards Framework. Semiquantitative RT-PCR. Average lead time: Call for Lead Time. Quantitative RT-PCR Analyses Total RNA of the collected samples were extracted using the RNeasy Plant Mini Kit (Qiagen, Hilden, Germany). For example, performance of OmniScript and SensiScript (both Qiagen) or AMV-derived RTases have been heavily influenced by experimental. 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. 95°C after the reverse transcription step. For MMLV Superscript, the pattern obtained was very faint at 200 ng of RNA, but indicated a >50-fold increase in detection levels for Sensiscript. # 18064 and 18080, respectively), and Omniscript® and Sensiscript® (Qiagen Cat. Technical Service; Customer Care . The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per. . 0 μg of RNA was reverse-transcribed using the Omniscript RT Kit (Qiagen Inc. An OmniScript is an omnichannel customer engagement and a business tool built on the Salesforce platform. SuperScript II, AMV-RT, Transcriptor RT, M-MuLV RT, M-MLV RT, Omniscript, DyNAmo, Statascript: Covariances of the RT efficiency were driven by target gene or total RNA concentration: Enzyme differences less important than diversity in gene-specific RT reproducibility: Bustin et al. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. The Sensiscript Reverse Transcriptase (RT) Kit is ideally suited for highly sensitive applications using very small amounts of RNA (less than 50 ng), such as single-cell RT-PCR and analysis of biopsies and LMD samples. . This kit is ideal for analyzing low input RNA samples. 1 mL RNase-free water. Please see the Omniscript Reverse Transcription Handbook for protocols. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript RT Kit. Migrate to Standard OmniStudio. 1. Kit contents: Qiagen Omniscript RT Kit, 50 rxns, RNA Template Sample, Reverse Transcription Enzyme Activity, High cDNA Yields Due to High-affinity Enzyme, Fast and Easy Procedure, For Reverse Transcription of 50ng to 2micro g RNA/Reaction for end-point PCR, Includes 200U Omniscript Reverse. DSL Modem Product. 1 ml 1. QIAstock. Omniscript RT Kit. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRQuantiTect RT Mix contains an optimized Omniscript and Sensiscript blend. PCR reactions were performed to determine Clk 1 mRNA levels using JumpStart Ready-Start amplification mix (Sigma) and previously published primers. A 20 μl RT reaction included 2 μg Universal reference RNA (Stratagen), 1 μM Oligo-dT primer, 2 μl of 10× RT buffer, 0. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. ,. Please see the Omniscript Reverse Transcription Handbook for protocols. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. File size. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. The rela‑ tive gene expression levels were normalized to GAPDH and calculated using the 2‑ΔΔCq method. Heat-mediated activation of these enzymes enables reaction setup at room temperature. CNV qPCR Assays & Arrays;. The QIAGEN One-Step Ahead RT-PCR Kit contains a blend of Sensiscript and Omniscript Reverse Transcriptases, a well-balanced combination of Taq DNA Polymerases and a proofreading enzyme. Yet, the RT reactions for standard curves constructed using Sensiscript and Omniscript produced reliable Cts at target concentrations as low as 10 3 copies/μl, similar to that observed for SSIV and SSIII (except for Sensi-RH: lower limit at 10 4 copies/μl). 1 ml 4 x. USD $1207. (B) Plasmid map of the predicted Omniscript RT Kit expression plasmid which was identified as the source of the EIAV pol. RT-PCR. Get Adobe Reader. 1 ml 1. 0 µl 5 mM dNTP (final concentration of 500 µM), 2. For one-step RT-PCR, the optimized Omniscript and Sensiscript blend for the reverse-transcription step further enhances sensitivity. The RNA yield and quality were determined using a NanoDrop system (ND-1000; Thermo Fisher Scientific). OmniScript は、お客様がビジネスプロセスを完了するためのガイド付きパスを提供し、シームレスなカスタマーエクスペリエンス (これは 常に 好ましいことです) を実現するため. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. RT-PCR assays were performed as previously described (Rosciszewski et al. Omniscript Reverse Transcriptase is a recombinant heterodimeric enzyme expressed in E. g. Genomic DNA contamination in RNA samples is effectively. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. Pharmaceutical Industry. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. 2800. USD $1207. Investor Relations Contacts. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor RNA, is there a difference in selecting RT enzymes with and without RNase H (e. For example, you can create an OmniScript t. Two micrograms of total RNA was reverse-transcribed using Omniscript RT kit (Qiagen). 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. (2013) NA mRNA invA-R García et al. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. V. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniScripts. DSL Service Product. Please see the Omniscript Reverse Transcription Handbook for protocols. Isolation and cloning of random PCR products. Time step T0 contained the reverse transcription mix (Omniscript RT Kit) without performing reverse transcription, T1 after the reverse transcription, and T2 was after a multiple displacement. When the reaction is heated to 50°C, the RT-blocker dissociates from the RT enzymes, rendering them fully active. For reverse transcription of 50 ng to 2 micro g RNA per reaction for end-point PCR. 2 μg of total RNA was used for reverse transcriptase synthesis using the. CNV qPCR Assays & Arrays;. After reverse transcription by Omniscript and Sensiscript Reverse Transcriptases, reactions are heated to 95°C for 15 minutes to activate HotStarTaq DNA Polymerase and toFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript®andSensiscript®RT WiderangeofRNAamounts(1pg–2µg) Highsensitivity OneStepRT-PCRBuffer Minimaloptimizationneeded. g. Reverse transcription is a powerful tool for creating complementary DNA (cDNA) from RNA, which can then be used in a variety of downstream applications for studying RNA. 205110, 205111and 205113) should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer. For 50 reverse-transcription reactions: 200 units Omniscript Reverse Transcriptase, 150 µl 10x Buffer RT, 100 µl dNTP Mix (contains 5 mM each dNTP), 1. 1 ml 4. 4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. Omniscript® Reverse Transcriptaseis designed for efficient and sensitive reverse transcription with 50 ng – 2 µg RNA. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. 1 ml RNase-free water 205111 询价 Reverse transcription and quantitative real-time PCR (RT-qPCR). Reverse transcription yields could vary among the reverse transcriptases in a highly gene-dependent way as a. Vlocity Intelligence. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. English. 1 ml 4. After the cDNA was diluted 1:3 in nuclease-free water, qRT-PCR was performed using the custom-designed primers (Biomers, Ulm, Germany) in Table 1 and the Platinum SYBR Green qPCR SuperMix-UDG kit (Invitrogen), or TaqMan Gene Expression Assays and. The commercial RT systems tested were Sensiscript, Omniscript, SuperScript II, SuperScript III, and PowerScript. Reverse transcription with AMV, MMLV, and Omniscript was performed at 37 °C, whereas with cAMV, Improm-II, and MMLVH it was performed at 45 °C, and with ThermoScript and SuperScript, it was performed at 50 °C. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. The QIAGEN OneStep Ahead RT-PCR Kit is designed for easy and sensitive one-step RT-PCR using any RNA template. reverse transcription of RNA amounts greater than 50 ng, and Sensiscript is optimized for use with very small amounts of RNA (<50 ng). Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. This product is not intended for the diagnosis, prevention, or treatment of a disease. 1 ml 4. RNA, using the Omniscript RT kit (Qiagen Inc. coli. OmniStudio Standard. These elements are placed in a specific sequence for the workflow. 1 ml 4. Omniscript, SuperScript III and PowerScript (P < 0. At this reaction temperature, common restrictions of RT-PCR are removed, simplifying assay design and eliminating compromises. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Enzyme activity: Reverse tran…The Sensiscript Reverse Transcriptase (RT) Kit is ideally suited for highly sensitive applications using very small amounts of RNA (less than 50. Unit definition: 2 Products found Omniscript RT Kit For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Sensiscript RT Kit For reverse transcription of less than 50 ng RNA per reaction for end-point PCR Discover the products and protocols you need Plan your entire experiment based on research interests. It uses PrimeScript Reverse Transcriptase, which features excellent extension. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRExamine OmniScript Elements. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR Balanced mixture of enzymes with optimized reverse-transcription buffer The QIAGEN One-Step RT-PCR Kit provides a blend of Sensiscript and Omniscript Reverse Transcriptases, HotStarTaq DNA Polymerase, QIAGEN OneStep RT-PCR Buffer, a dNTP mix, and Q-Solution, a novel additive that enables efficient amplification of “difficult” (e. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFAQ. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRNxtScript 2G RT is the latest member of the family and pushes the temperature of the RT step to new levels: no loss of activity even at 70°C. Both. , Valencia, CA). Reverse transcription reaction conditions RT kits used in this experiment were chosen to represent the most commonly used enzymes in the field (Fig. 3. Genomic DNA contamination in RNA samples is effectively eliminated by gDNA Wipeout Buffer. This kit's protocol is simple and suitable for high throughput. ZERO BIAS - scores, article reviews, protocol. Commercial Partner and Distributor Solutions. Both enzymes exhibit a high affinity for RNA, facilitating transcription through secondary structures that may inhibit other reverse transcriptases. It allows for the efficient labeling of a wide range of starting RNA concentrations and. 5 mM each dNTP, 10 U of RNase inhibitor, 4 U of Omniscript Reverse Transcriptase, and. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. In order to measure this difference on a large spectrum of background RNA, given that DNA and RNA concentrations are important influencing factors [ 8 - 10 ], the study used total RNA extracted from the same testis tissue (Methods). Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. Omniscript is designed for reverse transcription of RNA amounts greater than 50 ng, andFor reverse transcription of 50 ng to 2 μg RNA per reaction fork end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRExperimental validation. To understand the role of the colonic extracellular calcium-sensing receptor (CaSR) in calcium chemoprotection against colon cancer, we activated the CaSR with 5 mM Ca2+ on HT-29 cells, an adenocarcinoma cell line. The OneStep Ahead RT Mix contains an RNase inhibitor and a blend of Omniscript® and Sensiscript® reverse transcriptases in combination with an RT-blocker for heat-mediated activation of the reverse-transcription step. Sensiscript RT is optimized for use with very small amounts of RNA (<50 ng), such as for single-cell RT-PCR or analysis of small biopsies. Omniscript Reverse Transcriptase is a new, unique enzyme, different from the reverse transcriptases of Moloney murine leukemia virus (MMLV) or avian myeloblastosis virus. QIAGEN® OneStep RT-PCR Kit For fast and successful one-step RT-PCR The QIAGEN® OneStep RT-PCR Kit is designed for easy and sensitive one-step RT-PCR using any RNA template. The cDNA was generated using the Omniscript RT kit (Qiagen) with random hexamers (Roche Products, United States). reverse transcription, and quantitative PCR to work optimally in archived clinical samples. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript® Reverse TranscriptionFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR3 min read. PCR was performed on an ABI 7900HT using TaqMan® Universal PCR Master Mix, No Amperase® UNG (Life Technologies, Carlsbad, CA) or. Workflow Configurator. 1 ml RNase-free water 205111 询价For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRHigh cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR4 Omniscript Reverse Transcription Handbook 10/2010 Kit Contents Omniscript RT Kit (10) (50) (200) Catalog no. Total RNA from flag leaves of mature rice plants was isolated using the RNeasy Plant Mini Kit (Qiagen, Venlo, The Netherlands) and reversely transcribed using Omniscript RT Kit (Qiagen). Both biological and technical triplicates were run. Bioz Stars score: 96/100, based on 1 PubMed citations. IL-10 was demonstrated to be critical for suppressing autoimmunity and inflammatory responses ( 40 – 43 ), and whether Th17 express IL-10 is. using an Omniscript reverse transcriptase kit (Qiagen, Valencia, CA) in a 20- l reaction mixture, containing 200 ng of total RNA and specific primers, at 37°C for 1 h. RNA from Svalbard ptarmigan brain samples was extracted using TriReagent (Sigma-Aldrich) and the extracted RNA was converted into cDNA using the Omniscript RT kit from QIAGEN. There-fore, 2,500 copies (1 fg) was chosen as the relatively small. For MMLV Superscript, the pattern obtained was very faint at 200 ng of RNA, but indicated a >50-fold increase in detection levels for Sensiscript. A PCR was conducted using standard PCR methods (primers are available on request). 47 / EA. In order to measure this difference on a large spectrum of background RNA, given that DNA and RNA concentrations are important influencing factors [ 8 – 10 ], the study used total RNA extracted from the same testis tissue (Methods). Reactions were incubated at 65°C for 5 min, then 100 U RT enzyme was added and incubated at 37°C for 60 min and 75°C for 5 min. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROmniscript Quantiscript 37 NA mRNA Random hexamers Paulin et al. RT reactions containing 4 ng (Sensiscript) or 16 ng of total RNA (Omniscript) generated DD patterns indistinguishable from the 200 ng pattern. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCROne-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. C, Real-time RT-PCR for IL-17 and IL-10 expression in wild-type, DKO, and TKO CD4 + CD25 − T cells after 2 d of culture with anti-CD3 and anti-CD28, combined with IL-6, IL-27, or IL-6 plus IL-27. RT-PCR stands for Reverse Transcription PCR or Reverse Transcriptase PCR. Kit contents: Qiagen Omniscript RT Kit, 50 rxns,. At higher temperatures, RNA secondary structures unravel and no longer. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR High cDNA yields due to high-affinity enzyme Sensitive detection of as few as 10 copies of template No additional RNase H digestion step required Fast and easy procedure with no tedious pipetting steps The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with. The forward primer (5′ to 3′) GAAGCCGCTTGGAATAAGGC and the reverse primer (5′ to 3. All reactions took place for 1h at 37°C in a final volume of 40 μl containing 1 μg total RNA in the presence of 20 units RNase inhibitor (RNasin® Ribonuclease Inhibitor, Promega), 1 μg random hexamers (Microsynth), 4 μl 10. A unique enzyme combination and specially developed reaction buffer ensure efficient, highly specific reverse transcription and PCR in one tube, without the need for. The purification steps can be performed as described in the protocol using Qiagen’s PCR purification kit. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReverse transcription is described based on the Omniscript ® Reverse Transcription Kit (Qiagen). The assays were run under the following conditions. A high affinity for RNA allows Omniscript Reverse Transcriptase to provide superior performance compared with other reverse transcriptases, delivering higher sensitivity in RT-PCR, even with low. Martin Bilban, MSc PhD Student Quaranta Lab Department of Cell Biology The Scripps Research Institute The Omniscript RT Kit includes Omniscript Reverse Transcriptase, dNTPs and an optimized reaction buffer. Subsequent PCR was performed with primers (Sigma-Aldrich) based on the Icelandic rock ptarmigan genome and Taq DNA polymerase (QIAGEN). Data Migration with Build Tool. Probe-Based One-Step qRT-PCR; Probe-Based qPCR; Reverse Transcription & cDNA Synthesis for qPCR; SYBR Green- or Dye-Based One-Step qRT-PCR; SYBR Green- or Dye-Based qPCR; qPCR Assays & Instruments. Product Description. The Omniscript Reverse Transcription (RT) Kit is specially designed for reverse transcription with any amount of RNA from 50 ng to 2 μg per reaction. Probably the most important being that the OmniScript RT allows for the labeling of even very small amounts of RNA (i. 1 ml 1. One-Step RT-PCR; Reverse Transcription & cDNA Synthesis; Real-Time PCR Enzymes & Kits. Please see the Omniscript Reverse Transcription Handbook for protocols. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRSensiscript Reverse Transcriptase is specially designed for highly sensitive reverse transcription with small amounts of RNA (<50 ng). Ordering Support. Reverse transcriptase is a multifunctional enzyme with three distinct enzymatic. For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRFor reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCRReal-time RT-PCR A OneStep RT-PCR Kitb was used with a 25-#l reaction volume. 205110 205111 205113 Number of reactions* 10 50 200 Omniscript Reverse Transcriptase 40 units 200 units 800 units Buffer RT, 10x 30 µl 150 µl 4 x 150 µl dNTP Mix, 5 mM each 20 µl 100 µl 4 x 100 µl RNase-Free Water 1. 381KB. The reverse transcription (RT) step conditions for all primer sets were 30 min at 50 C and 15 min at 95 C. Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability. Nine parallel large-scale GoScript™ RT reactions were performed, containing log-fold variations of input human reference total RNA (10ng/µl to 0.